Journal: SpringerPlus

Article Title: Molecular sexing of threatened Gyps vultures: an important strategy for conservation breeding and ecological studies

doi: 10.1186/2193-1801-1-62

Figure Lengend Snippet: Sequence alignment of CHD-Z and CHD-W allele sequences amplified by Griffith’s universal CHD primer pair P2/P8 in four species of vultures ( Gyps bengalensis , Gyps indicus , Gyps himalayensis , Aegypius monachus ) by ClustalW (MegAlign DNAstar). The sequences of primers P2, P8, NP, MP, ZW common primer and probe, W -specific primer and probe recognition sites; Bam HI and Rsa I restriction sites are boxed. (−−) Dashed lines indicate sequence similarity. A .) Alignment of CHD-Z sequences. B .) Alignment of CHD-W sequences.

Article Snippet: The PCR reaction mixture in a 20 μl volume consisted of 0.4 μM each of Griffith’s universal CHD primer pair P2/P8, 50–100 ng genomic DNA, 0.2 mM of each dNTP, 20nM each of W -specific and ZW common probes and 1 U of Taq DNA polymerase (Bangalore Genei, India).

Techniques: Sequencing, Amplification

Journal: SpringerPlus

Article Title: Molecular sexing of threatened Gyps vultures: an important strategy for conservation breeding and ecological studies

doi: 10.1186/2193-1801-1-62

Figure Lengend Snippet: Predicted gel pattern for analysing various sex identification methods (size in bp)

Article Snippet: The PCR reaction mixture in a 20 μl volume consisted of 0.4 μM each of Griffith’s universal CHD primer pair P2/P8, 50–100 ng genomic DNA, 0.2 mM of each dNTP, 20nM each of W -specific and ZW common probes and 1 U of Taq DNA polymerase (Bangalore Genei, India).

Techniques: Multiplex Assay, Amplification

Journal: SpringerPlus

Article Title: Molecular sexing of threatened Gyps vultures: an important strategy for conservation breeding and ecological studies

doi: 10.1186/2193-1801-1-62

Figure Lengend Snippet: Gel view for molecular method for sex identification of Gyps bengalensis using Griffith’s universal CHD primer pair P2/P8 PCR based methods: P2/P8 PCR (L1), Bam HI digest of P2/P8 amplicon (L2). Rsa I digest of P2/P8 amplicon (L3), ARMS PCR (L4), P2/ ZW -common PCR (L5), and P2/ W -Specific PCR (L6). M: 100 bp DNA ladder; NTC: No template control. The PCR products and restriction digests were resolved in run 3% agarose gel electrophoresis and stained with ethidium bromide A . Anatomically confirmed female (P10). B . Anatomically confirmed male (P33).

Article Snippet: The PCR reaction mixture in a 20 μl volume consisted of 0.4 μM each of Griffith’s universal CHD primer pair P2/P8, 50–100 ng genomic DNA, 0.2 mM of each dNTP, 20nM each of W -specific and ZW common probes and 1 U of Taq DNA polymerase (Bangalore Genei, India).

Techniques: Amplification, Agarose Gel Electrophoresis, Staining

Journal: SpringerPlus

Article Title: Molecular sexing of threatened Gyps vultures: an important strategy for conservation breeding and ecological studies

doi: 10.1186/2193-1801-1-62

Figure Lengend Snippet: Gel view for molecular methods for sex identification using post-mortem and live bird samples of vultures by Griffith’s universal CHD primer P2/P8 based PCR methods- PCR- RFLP using Bam HI ( A ), PCR-RFLP using Rsa I ( B ), ARMS-PCR ( C ), P2/ ZW -Common PCR ( D ), P2/ W -Specific PCR ( E ). M: 100 bp DNA ladder; NTC: No template control. The PCR products and restriction digests were resolved on 3% agarose gel and stained with ethidium bromide.

Article Snippet: The PCR reaction mixture in a 20 μl volume consisted of 0.4 μM each of Griffith’s universal CHD primer pair P2/P8, 50–100 ng genomic DNA, 0.2 mM of each dNTP, 20nM each of W -specific and ZW common probes and 1 U of Taq DNA polymerase (Bangalore Genei, India).

Techniques: Agarose Gel Electrophoresis, Staining